ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary An antiserum against the β-amylase from Bacillus cereus BQ10-S1 Spo II was prepared using rabbits. The antiserum obtained was confirmed to form a specific immunoprecipitate with the purified β-amylase and showed a single band of protein with a molecular weight of 6.0x104 on the nitrocellulose sheet by the Western-Blotting method. The antiserum showed a precipitin line with the β-amylase from B. megaterium strain no. 32 by the Ouchterlony technique. However, the spur was formed on the Ouchterlony plate between the line of immunoprecipitin of the β-amylase from B. cereus BQ10-S1 Spo II and that from B. megaterium strain no. 32. On the other hand, no immune reaction occurred with the β-amylase from B. polymyxa no. 72 and those from higher plants such as soybean and barley. B. cereus BQ10-S1 Spo II was found to secret β-amylase mainly from the mid to the late logarithnic phase of cell growth. With the use of antiserum, the amount of the β-amylase secreted was estimated to be about 52 μg/109 cells, that of the parent strain (B. cereus BQ10-S1) about 14 μg/109 cells. These quantities of β-amylase corresponded in each case with enzyme productivity of the two strains (about 1,100 U/ml and 270 U/ml).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249985
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