ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
We have succeeded in developing a fully automated machine to handle the Sanger dideoxy sequencing reactions. The robot is constructed of a pipetter unit, reaction unit, tip storage unit, and reagent storage unit. The pipetting precision is found to be 0.037 in terms of standard deviation at the dispensing volume of 0.95 μl, and the cross contamination between different sample solutions in the course of pipetting is reduced to 0.005 μl. Satisfactory results are obtained in temperature control and in the suppression of liquid evaporation, and a precision equivalent to a high level of manual manipulation is realized in DNA sequencing analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1141168
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