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  • Articles  (92)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 160 (1998), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The human transferrin receptor, a type II plasma membrane protein which mediates iron transport in human cells, was expressed in the yeast Saccharomyces cerevisiae. The transferrin receptor synthesized by yeast cells was posttranslationally modified comparable to the native receptor with respect to glycosylation and dimer formation. The location of the expressed receptor in the yeast plasma membrane indicates that the targeting of this type II membrane protein shares similar mechanisms in yeast and mammalian cells. The yeast-expressed transferrin receptor showed binding activity towards its natural ligand, transferrin in an ELISA binding assay.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 21 (1997), S. 0 
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Glycerol is the main compatible solute in Saccharomyces cerevisiae. It is accumulated intracellularly when cells are exposed to decreased extracellular water activity. In general, increased intracellular accumulation of a solute may be caused by enhanced production, restricted dissimilation, increased retention by the plasma membrane and increased uptake from the medium. In this review, we evaluate current knowledge concerning mechanisms leading to the accumulation of glycerol in osmotically stressed cells of S. cerevisiae at the molecular and metabolic levels. An overview of glycerol metabolism in S. cerevisiae is provided.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 75 (1990), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 237-241 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In order to establish a transformation system for P. chrysogenum autonomously replicating vectors were constructed using mitochondrial DNA sequences from the fungus. A physical map of the mt DNA of a production strain was established using ten different restriction enzymes. Unexpectedly, the mt DNA of this strain proved to be significantly smaller than that of a second strain from a culture collection (27 kb versus 49 kb). Various fragments representing about 71% of the 27 kb mt DNA were cloned and, at first, preselected for replicating activity in an intermediate host (Saccharomyces cerevisiae). Two of these fragments also promoted autonomous replication in P. chrysogenum, which was confirmed by isolation of bulk DNA and transfer into E. coli. For selection of transformants in P. chrysogenum the prokaryotic kanamycin resistance gene was used which increased about twofold the resistance against G418.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 12 (1987), S. 291-295 
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Group II intron ; Lariat RNA ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In senescing strains of the filamentous ascomycete Podospora anserina, the first intron (il) of the mitochondrial gene for cytochrome-c-oxidase subunit I (CO I), a group II intron, accumulates as a circular mitochondrial plasmid (plDNA). In both juvenile and senescent wild type strain s two highly abundant transcripts were detected homologous to il and plDNA. In this report we show that these RNAs are identical molecules having different conformations: the first is a lariat, the second a corresponding linear molecule probably resulting from breakage of the branched circular form. Our findings suggest that the transcripts arise from processing of CO I pre-mRNA, including il, rather than from transcription of the excised plasmid. The significance of the lariat RNA concerning plDNA amplification via a postulated reverse transcription mechanism is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 16 (1989), S. 57-60 
    ISSN: 1432-0983
    Keywords: Podospora anserina ; Immortal mutant ; Transformation ; Benomyl resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Podospora anserina immortal mutant incoloris, vivax was transformed to benomyl resistance with a β-tubuline gene from a resistant Neurospora crassa strain. The transforming plasmid was integrated into the genome of the transformants, and subsequent Southern analysis and retransformation experiments provided no evidence for autonomous replication. Non-homologous integration was demonstrated in some of the transformants. Resistance to benomyl varied widely among the transformants and was conserved after the transformants were grown on non-selective medium.
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  • 7
    ISSN: 1432-0983
    Keywords: Schizosaccharomyces pombe ; S. cerevisiae adc1 promoter ; S. pombe adh1 promoter ; Aminoglycoside phosphotransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro aminoglycoside phosphotransferase activity. On the other hand, the heterologous promoter of the Saccharomyces cerevisiae alcohol dehydrogenase I gene (adc1) is shown to be a weak promoter in Schizosaccharomyces pombe, though its activity is significantly enhanced in cells grown on glycerol as a carbon source. This system for selection and detection of promoter-active sequences may provide a useful basis for the analysis of promoter elements in fission yeast.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 387-391 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In order to evaluate the potential of Zymomonas mobilis and Saccharomyces cerevisiae for biotechnological ethanol production both microbes were grown aerobically and anaerobically at different glucose concentrations. The optimal values for ethanol production related to the theoretical values are 94% for Zymomonas and 88% for Saccharomyces, both under anaerobiosis. The corresponding figures for biomass are 2.5 g/l and 6.5 g/l and for total acid content 16 mM/l and 12 mM/l respectively: for other byproducts (such as aldehydes, ketones, esters) 330 mg/l and 280 mg/l respectively. For industrial fermentation Z.mobilis seems to be inferior to S.cerevisiae, because the advantage of higher ethanol and the lower biomass production of the bacterium is disadvantaged by the decrease in pH from 6.3 to 3.3 during yeast fermentation thus making sterilization of the medium unnecessary. The formation of acids and other byproducts may be neglected in practice, since in contrast to previous assumptions they are easy to separate during the rectification.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 30 (1989), S. 343-350 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary By developing an efficient transformation system it was possible to reintroduce two different glucose dehydrogenase genes of Bacillus megaterium into this host. These genes were previously cloned, sequenced and expressed in Escherichia coli. Since the expression of one of these genes (gdhA) turned out to be extremely high in B. megaterium, an expression system for genes from closely and distantly related organisms using the controlling region of the gdhA gene was developed.
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  • 10
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The neo genes of Tn5 and Tn903 (Tn601) coding for amigoglycoside phosphotransferase type II and type I, respectively, were joined to the yeast adc 1 promoter and trp1 terminator and introduced into yeast (Saccharomyces cerevisiae) cells. Transformants were obtained by direct selection for G418 resistance. Plasmids containing the Tn5 neo gene induced antibiotic resistance only at low frequency, whereas colonies transformed with the Tn903 neo gene could be selected at high frequency (300–400 transformants/μg plasmid DNA). The resistance threshold of transformed strains was increased to 30 mg G418/ml by both genes and high level expression of the bacterial genes in yeast could be shown using an in vitro phosphotransferase assay. The results indicate that this system can be used for high frequency transformation of wild-type strains and might in addition be used for the identification and isolation of promoter-active sequences.
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