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  • Articles  (23)
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  • Articles  (23)
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  • 11
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    Chromosome VIII disomy influences the nonsense suppression efficiency and transition metal tolerance of the yeastSaccharomyces cerevisiae (2015)
    Wiley
    Details
    Publication Date: 2015-05-06
    Print ISSN: 0749-503X
    Electronic ISSN: 1097-0061
    Topics: Biology
    Published by Wiley
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    PAPER CURRENT
  • 12
    Electronic Resource
    Electronic Resource
    Triallelic Complementation and the Subunit Structure of Enzymes (1969)
    [s.l.] : Nature Publishing Group
    Nature 222 (1969), S. 1078-1079 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Complementation maps are helpful in estimating the degree of structural damage to the protein subunits synthesized under the control of the complementing alleles. Modern theory of interallelic complementation1 arises from data obtained chiefly from investigations using microorganisms. All ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2221078a0
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  • 13
    Electronic Resource
    Electronic Resource
    Hybridization and cytoduction among yeast cells of the same mating type (1987)
    Springer
    Current genetics 12 (1987), S. 511-517 
    Details
    ISSN: 1432-0983
    Keywords: Mating-type switching ; Cytoduction ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Use of a selective system for cytoduction in Saccharomyces cerevisiae allowed us to monitor hybrid formation and to clone the haploid nuclei of cells which have participated in illegitimate matings: a × a, α × α. Our approach has made it possible to select nuclei with mating-type switches and mutations within the MAT locus. It was shown that matings in α × α crosses often proceed through nonheritable genetic changes located within chromosome III. We suggest that these non-heritable genetic changes are due to premutational lesions, expressed phenotypically as transient α-matingtype. After a mating event these lesions are either repaired or converted to true mutations within the MAT locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419560
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  • 14
    Electronic Resource
    Electronic Resource
    Drug-dependent mutants in yeast Saccharomyces cerevisiae (1983)
    Springer
    Current genetics 7 (1983), S. 357-362 
    Details
    ISSN: 1432-0983
    Keywords: Cycloheximide dependent mutants ; Ribosomal suppressors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutations in sup1 and sup2 genes may cause cycloheximide-dependent growth in yeast Saccharomyces cerevisiae. Two classes of such mutants are described in the paper: 1) high temperature sensitive mutants, which do not express their sensitivity to nonpermissive temperature in the presence of cycloheximide (conditionally dependent) and 2) mutants unable to grow in the absence of the drug (true dependent). Some of the mutants of both classes express dependence toward another antibiotic — trichodermine. The binding of H3-labelled cycloheximide studied by equilibrium dialysis has demonstrated that both 80S ribosomes and 60S subunits isolated from conditionally dependent mutant showed a higher affinity for the drug compared to that of a parent strain. The number of binding sites per ribosome or per 60S subunit in the cycloheximide dependent mutant remains unchanged. Circular dichroism spectra of a mutant ribosomes in the presence as well as in the absence of antibiotic revealed that sup1 and sup2 mutations alter conformation of the yeast cytoplasmic ribosomes. The binding of cycloheximide to mutant ribosomes induces a conformational shift, which presumably compensates for their functional defect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445875
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  • 15
    Electronic Resource
    Electronic Resource
    The effect of paromomycin on the expression of ribosomal suppressors in yeast (1982)
    Springer
    Current genetics 5 (1982), S. 149-152 
    Details
    ISSN: 1432-0983
    Keywords: Ribosomal mutants ; Translational ambiguity ; Recessive suppression ; Paromomycin action
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of the yeast Saccharomyces cerevisiae carrying ribosomal suppressor mutations in either sup1 or sup2 genes express a higher sensitivity to paromomycin — aminoglycoside antibiotic known to induce translational errors in eukaryotes. Paromomycin also induces a phenotypic suppression of all three types of nonsense mutations (ochre, amber and opal), missense mutations and frame-shift mutations. The influence of paromomycin on the activity of ribosomal suppressors has at least two aspects: (1) the drug increases translational ambiguity in sup1 and sup2 mutants in vitro and (2) it induces the alteration (extension or restriction) of sup 1 or sup2 suppression spectra in vivo. A modification of selectivity of the mutant ribosomes towards different tRNAs in the presence of paromomycin is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365706
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  • 16
    Electronic Resource
    Electronic Resource
    Rapid genetic test for discrimination between haploid and polyploid transformants in Saccharomyces (1984)
    Springer
    Current genetics 8 (1984), S. 341-344 
    Details
    ISSN: 1432-0983
    Keywords: Spheroplast and lithium chloride transformation ; Mitotic homozygotization ; Polyploid transformants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spheroplast fusion induced by polyethylene glycol often occurs during transformation of yeast spheroplasts. It leads to the appearance of polyploid transformants. We have developed a method of discriminating between haploid and polyploid transformants, based on qualitative comparison of the frequency of mitotic homozygotization for a recessive marker, lys2, in diploid and polyploid hybrids of transformants crossed with a tester haploid bearing lys2. We have used this method to compare the frequency of appearance of polyploids during transformations of spheroplasts and of intact cells using lithium chloride in the strain DC5. Polyploids constitute from 15% to 74% of transformants after transformation of spheroplasts. In lithium chloride transformation polyploids arose in only one of four experiments and constituted 9% of transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419822
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  • 17
    Electronic Resource
    Electronic Resource
    Recessive super-suppression in yeast (1974)
    Springer
    Molecular genetics and genomics 129 (1974), S. 105-121 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mechanism of recessive nonsense-suppression was studied in haploid strains of yeast Saccharomyces cerevisiae which were temperature-sensitive due to recessive suppressors. It was demonstrated that these strains carry a defect in protein synthesis leading to the temperature sensitivity of translation. Examination of separate translation events in parent sup - and suppressor-carrying strains has shown that recessive suppression is most likely caused by a mutant protein participating in or affecting the termination of peptide chains on ribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268625
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  • 18
    Electronic Resource
    Electronic Resource
    Relationship between cytoplasmic and mitochondrial apparatus of protein synthesis in yeast Saccharomyces cerevisiae (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 172-174 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A conditional respiratory deficiency in yeast Saccharomyces cerevisiae is expressed as a result of a nuclear mutation in sup1 and sup2 genes (II and IV chromosomes, respectively), coding for a component of cytoplasmic ribosomes (Ter-Avanesyan et al. 1982). One such strain is studied here in detail. The strain is temperature-dependent and expresses a respiratory deficient phynotype at 20° C but not at 30° C. Moreover, the strain is simultaneously chloramphenicol-dependent and is able to grow on media containing glycerol or ethanol as a sole carbon source only in the presence of the drug. Chloramphenicol has a differential effect on protein synthesis in mitochondria of the parent strain and the mutant. Since chloramphenicol is a ribosometargeting antibiotic we suggest that the differential effect of the drug on parent and mutant mitochondrial protein synthesis is due to the altered properties of mito-ribosomes of the mutant compared to those of the parent strain. Mitochondria of the mutant synthesize all the mitochondrially encoded polypeptides, however, in significantly lowered amounts. A suggestion is put forward for the existence of a common component (a ribosomal protein) for mito and cyto-ribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326073
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  • 19
    Electronic Resource
    Electronic Resource
    Super-suppressor induced interallelic complementation (1966)
    Springer
    Molecular genetics and genomics 98 (1966), S. 375-384 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In yeast the dominant super-suppressorS 5 has a distinct expression in heterozygotes depending on the particular combination of alleles at thead 1 orad 2 loci. If thead 1 combination is represented by two suppressible alleles, the phenotype of diploid is wild. If thead 1 combination consists of a suppressible and a non-suppressible allele the phenotype of the diploid is partially mutant. Such a difference in the manifestation of suppressor depending on the combination of alleles is more pronounced in the case ofad 2 mutations. In the case when bothad 2 alleles are suppressible, the diploid is prototrophic, but when only one allele is suppressible, the diploid is an adenineless auxotroph as a rule. This type ofS 5-effect gave us the possibility to study interallelic complementation atad 2 locus in presence of the super-suppressor. It was shown that some combinations of noncomplementing alleles do complement as a result of suppression. Comparison of the two complementation maps with and without suppressor is made for thead 2 locus. The mechanisms of the phenomena and of super-suppression are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00895265
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  • 20
    Electronic Resource
    Electronic Resource
    Nonsense-missense suppression in yeast (1971)
    Springer
    Molecular genetics and genomics 113 (1971), S. 302-307 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A dominant suppressor has been discovered which suppresses polarity type mutations, noncomplementing ones and those belonging to short nonpolar complementation groups at the ad 2 locus of S. cerevisiae. The suppressor translates two types of nonsense previously established at ad 2. These data together with theoretical consideration of relationships between nonsense-translating anticodons and different codons make it possible to suggest a mechanism for the observed suppression. According to this mechanism two nonsense types of ad 2 are identified as amber and ochre codons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272330
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