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  • Articles  (41)
  • Biology  (41)
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  • Articles  (41)
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  • 1
    Electronic Resource
    Electronic Resource
    Transgenic avidin maize is resistant to storage insect pests (2000)
    [s.l.] : Nature America Inc.
    Nature biotechnology 18 (2000), S. 670-674 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Avidin is a glycoprotein found in chicken egg white, that sequesters the vitamin biotin. Here we show that when present in maize at levels of ≥100 p.p.m., avidin is toxic to and prevents development of insects that damage grains during storage. Insect toxicity is caused by a biotin deficiency, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/76531
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  • 2
    Electronic Resource
    Electronic Resource
    Increased Luminance in the Direction of Reflex Reflexion—a Recently Observed Natural Phenomenon (1969)
    [s.l.] : Nature Publishing Group
    Nature 224 (1969), S. 1102-1102 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In 1962, Meacock et al.1 drew attention to the increased luminance of (smoked) magnesium oxide in the direction of reflex reflexion, when incident light is normal to the surface. A repeat of these experiments, in which the simple photo-multiplier was replaced by a Pritchard direct viewing ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2241102b0
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  • 3
    Electronic Resource
    Electronic Resource
    Presence of Istiophoridae along the South Atlantic and Mediterranean Coasts of Spain (1962)
    [s.l.] : Nature Publishing Group
    Nature 196 (1962), S. 495-496 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In October 1961 Dr. C. Richard Robins, of the Marine Laboratory of the University of Miami, confirmed, at Cadiz, the identification of nineteen of the specimens as T. albidus Poey (= Makaira albida (Poey) of some authors). Dr. Robins is investigating the other two specimens still further before ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/196495a0
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  • 4
    Electronic Resource
    Electronic Resource
    Light industry (1987)
    [s.l.] : Nature Publishing Group
    Nature 330 (1987), S. 290-291 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] EDWIN Land was born in Connecticut in 1909. Even as a youth he had decided he would achieve fame and fortune in the world of science and technology. After all, had not Thomas Edison, Henry Ford, Alexander Graham Bell and George Eastman - all self-made men, and all then still alive - succeeded by ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/330290a0
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  • 5
    Electronic Resource
    Electronic Resource
    Relation between Van der Waals' and Activated Adsorption on Chromium Oxide Gel (1933)
    [s.l.] : Nature Publishing Group
    Nature 132 (1933), S. 603-603 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In the absence of definite evidence to the contrary, it has always been assumed that Van der Waals' and activated adsorption take place independently of each other and are additive. Such, however, is not the case, for activated adsorption on a surface has been found to diminish Van der Waals' ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/132603a0
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  • 6
    Electronic Resource
    Electronic Resource
    Transgenic tobacco plants and their progeny derived by microprojectile bombardment of tobacco leaves (1990)
    Springer
    Plant molecular biology 14 (1990), S. 261-268 
    Details
    ISSN: 1573-5028
    Keywords: direct gene transfer ; gene rearrangements ; Nicotiana tabacum ; particle gun ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic tobacco plants and progeny carrying coding sequences for neomycin phosphotransferase II (NPTII) and beta-glucuronidase (GUS) were recovered following microprojectile bombardment of tobacco leaves. Transgenic plants were regenerated from bombarded leaf pieces of tobacco cvs. ‘Xanthi’ and ‘Ky 17’ which were cultured in the presence of 100 or 200 μg/ml kanamycin for six to eight weeks. Among 160 putative transgenic plants from at least 16 independent transformation events 76% expressed NPTII, and 50% expressed GUS. Southern analysis of plants expressing either one or both of the enzymes indicated DNA in high molecular weight DNA in 8 of 9 independent transformants analyzed. Two independent transformants and their progeny were analyzed in detail. Analysis of progeny for quantitative enzyme levels of NPTII and GUS, and Southern analysis of parents and progeny clearly demonstrated that the genes were transmitted to progeny. One transformant demonstrated Mendelian ratios for seed germination on kanamycin-containing medium while the other transformant had non-Mendelian ratios. DNA analysis of progeny indicate complex integration of the plasmid DNA, and suggest that rearrangements of this DNA has occurred. These results are consistent with other methods of direct DNA uptake into cells, and verify that the microprojectile bombardment method is capable of DNA delivery into intact plant cells which can give rise to transgenic plants and progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018566
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  • 7
    Electronic Resource
    Electronic Resource
    An efficient rinsing procedure for 10 mm cell culture inserts prior to coculture studies (1996)
    Springer
    Methods in cell science 18 (1996), S. 25-29 
    Details
    ISSN: 1573-0603
    Keywords: Co-culture ; Cell culture inserts ; Rinsing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A goal of our laboaatory is to define putative intercellular communication mechanisms between myofibers and adipocytes. In pursuit of this objective we established a co-culture system incorporating commercially available microporous cell culture inserts. These cell culture inserts provide separation of cells bathed in a common media environment. Isolation and evaluation of the conditioned media from such systems allows detection of autocrine or paracrine agent production by either cell type. However, the process of rinsing cells plated on inserts prior to initiating co-culture may damage insert integrity and may dilute (added) defined treatment medium by introducing residual rinse medium. We have developed a method to rinse microporous membrane inserts before the addition of defined treatment medium. This rinsing technique decreases damage to inserts and reduces the volume of residual medium on all physical aspects of the insert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00123520
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  • 8
    Electronic Resource
    Electronic Resource
    3H-methyl scopolamine binding to dispersed pancreatic acini (1981)
    Springer
    Cell & tissue research 220 (1981), S. 673-684 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas ; 3H-methyl scopolamine ; Amylase release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Maximal amylase release occurred with 10-5 M carbachol and slightly greater than half maximal response occurred with 3×10-7 M carbachol in dispersed pancreatic acini. The preparation released more than 45% of its initial amylase content after 60 min of maximal carbachol stimulation. Electron microscopy revealed depletion of zymogen granules and the presence of secretory material in the ductules after carbachol stimulation. At 37° C, maximal binding of methyl scopolamine occurred in about 45 min with 3×10-10 M 3H-methyl scopolamine. The dissociation constant for 3H-methyl scopolamine was 6.8×10-10 M and saturation occurred at 109 pm/g protein. The I.C. 50 for 3H-methyl scopolamine inhibition of carbachol-induced amylase secretion was 7 × 10-10 M.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210454
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  • 9
    Electronic Resource
    Electronic Resource
    Production of recombinant proteins in transgenic plants: Practical considerations (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 473-484 
    Details
    ISSN: 0006-3592
    Keywords: transgenic plants ; recombinant protein ; gene expression ; downstream processing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This review is based on our recent experience in producing the first commercial recombinant proteins in transgenic plants. We bring forward the issues that have to be considered in the process of selecting and developing a winning transgenic plant production system. From the production point of view, transcription, posttranscription, translation, and posttranslation are important events that can affect the quality and quantity of the final product. Understanding the rules of gene expression is required to develop sound strategies for optimization of recombinant protein production in plants. The level of recombinant protein accumulation is critical, but other factors such as crop selection, handling and processing of transgenic plant material, and downstream processing are equally important when considering commercial production. In some instances, the cost of downstream processing alone may determine the economic viability of a particular plant system. Some of the potential advantages of a plant production system such as the high levels of accumulation of recombinant proteins, glycosylation, compartmentalization within the cell, and natural storage stability in certain organs are incentives for aggressively pursuing recombinant protein production in plants. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 473-484, 1997.
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  • 10
    Electronic Resource
    Electronic Resource
    Processing of transgenic corn seed and its effect on the recovery of recombinant β-glucuronidase (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 44-52 
    Details
    ISSN: 0006-3592
    Keywords: β-glucuronidase ; recovery ; recombinant enzyme ; protein extraction ; transgenic corn ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The tools of plant biotechnology that have been developed to improve agronomic traits are now being applied to generate recombinant protein products for the food, feed, and pharmaceutical industry. This study addresses several processing and protein recovery issues that are relevant to utilizing transgenic corn as a protein production system. The gus gene coding for β-glucuronidase (rGUS) was stably integrated and expressed over four generations. The accumulation level of rGUS reached 0.4% of total extractable protein. Within the kernel, rGUS was preferentially accumulated in the germ even though a constitutive ubiquitin promoter was used to direct gus expression. Fourth-generation transgenic seed was used to investigate the effect of seed processing on the activity and the recovery of rGUS. Transgenic seed containing rGUS could be stored at an ambient temperature for up to two weeks and for at least three months at 10°C without a significant loss of enzyme activity. rGUS exposed to dry heat was more stable in ground than in whole kernels. The enzyme stability was correlated with the moisture loss of the samples during the heating. Transgenic seed was dry-milled, fractionated, and hexane extracted to produce full-fat and defatted germ fractions. The results of the aqueous extraction of rGUS from ground kernels, full-fat germ, and defatted-germ samples revealed that approximately 10 times more rGUS per gram of solids could be extracted from the ground full-fat germ and defatted-germ than from the kernel samples. The extraction of corn oil from ground germ with hot hexane (60°C) did not affect the extractable rGUS activity. rGUS was purified from ground kernels and full-fat germ extracts by ion exchange, hydrophobic interaction, and size exclusion chromatography. Similar purity and yield of rGUS were obtained from both extracts. Biochemical properties of rGUS purified from transgenic corn seed were similar to those of E. coli GUS. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 44-52, 1998.
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