ISSN:
1573-0972
Keywords:
Co-operative activities
;
Humicola grisea var thermoidea
;
thermophilic fungi
;
xylan-hydrolysing
;
xylanases
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A thermophilic fungus, Humicola grisea var thermoidea, produced in liquid culture two endoxylanases (1,4-β-d-xylan-xylanohydrolase, EC 3.2.1.8) with M r of 95 (Xyl I) and 13 (Xyl II) kDa. PAGE of the crude culture filtrate and of each fraction obtained by gel filtration produced three and one band, respectively. Cross-reaction of the culture filtrate and each fraction with polyclonal antibodies prepared against Xyl II produced two and one precipitin bands, respectively. Hydrolysis of wheat straw and rice husk xylan was maximal using a combination of Xyl I and Xyl II. The products formed after hydrolysis, xylo-oligosaccharides and traces of xylose, indicated an endotype enzyme action and the co-operative activities of the xylanases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327791
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