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  • virus resistance  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Combining a host gene and a tobacco vein mottling virus coat protein gene for broad and effective resistance to potyviruses in tobacco (Nicotiana tabacum L.) (1997)
    Springer
    Molecular breeding 3 (1997), S. 331-339 
    Details
    ISSN: 1572-9788
    Keywords: additive effect ; plant transformation ; transgenic ; virus resistance ; Virgin A mutant gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Over 100 transgenic tobacco lines in five genetic backgrounds were transformed with the tobacco vein mottling virus (TVMV) coat protein (CP) gene. Transgenic lines were initially tested for their reaction to inoculation with a TVMV systemic strain (TVMV-S) and a potato virus Y common strain (PVY-O). Of the 104 TVMV CP lines 60% were classified as resistant to PVY-O, whereas only 30% of these same lines were resistant to TVMV-S. A subset of six PVY-O-resistant transgenic lines and four control lines were tested for their reaction to a local isolate of TVMV, tobacco etch virus (TEV) and five isolates of PVY. The same ten lines were also tested for responses to a serial dilution of inoculum for two PVY isolates, PVY-KY1 and PVY-NN. Transgenic lines carrying an endogenous resistance gene known as Virgin A mutant (VAM) had greater resistance and a broader spectrum of resistance than did transgenic lines without the VAM gene. This additive effect of the endogenous resistance gene and coat protein-mediated resistance (CPMR) was not overcome by the highest inoculum concentration. The results indicate that the additive effect of the VAM gene and CPMR could extend the effectiveness of CPMR in controlling potiviruses. These findings could have important implications for plant improvement programs using CPMR against potyvirus diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009680929276
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  • 2
    Electronic Resource
    Electronic Resource
    Field resistance of transgenic burley tobacco lines and hybrids expressing the tobacco vein mottling virus coat protein gene (1997)
    Springer
    Molecular breeding 3 (1997), S. 319-330 
    Details
    ISSN: 1572-9788
    Keywords: additive effect ; coat protein-mediated resistance ; potyviruses ; virus resistance ; Virgin A Mutant gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifty transgenic lines expressing the tobacco vein mottling virus (TVMV) coat protein (CP) gene in five genetic backgrounds were evaluated under field conditions for response to mechanic inoculation with TVMV, tobacco etch virus (TEV) and potato virus Y (PVY). TVMV CP transgenic lines conferred resistance to TVMV, TEV and PVY under field conditions. Combining two strategies, coat protein-mediated resistance (CPMR) coupled with an endogenous resistance gene (Virgin A Mutant, VAM) significantly extended the range and magnitude of virus resistance and provided a potential valuable new source of protection against potyviruses. CP transgenic lines lacking the VAM gene had high resistance to TEV, medium resistance to PVY, and a recovery phenotype to TVMV. A series of hybrids involving transgenic lines were generated and tested under field conditions for response to virus inoculation. One copy of TVMV-CP gene presented in lines homozygous for the VAM gene provided effective resistance to all three potyviruses. These studies also suggested that selection of a suitable recipient genotype was critical and that field evaluation was necessary in order to select elite resistant transgenic lines. Engineering viral CP genes into genotypes possessing some level of virus resistance could be critical to achieve an effective level of resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009618931171
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    Paper (German National Licenses)
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