ISSN:
1573-4986
Keywords:
cryoprecipitable kappa light chain
;
tetra-antennary type oligosaccharide
;
light chain deposition disease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract In the light of the hypothesis that the kappa light chain accumulation in massive cutaneous hyalinosis (MCH) is related to an abnormal glycosylation pattern, we analyzed the oligosaccharide structures of the cold precipitable kappa light chains excreted in the urine of an MCH patient. The MCH kappa chains contain about 25% carbohydate. Concanavalin A-Sepharose affinity chromatography of the glycopeptides obtained from pronase digests showed that the bulk of the glycopeptides (82%) did not bind to the column; 16% had a weak affinity, and 2% a strong affinity. Methylation analyses indicated that the unbound fraction consisted mainly of tetra-antennary glycopeptides, but tri-antennary and bisecting structures were also found. Most of the weakly-bound glycopeptides had a biantennary carbohydrate structure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01048436
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