ISSN:
1573-0778
Keywords:
Alphitonia zizyphoides
;
biotechnology
;
cell survival
;
lymphoid cells
;
plating efficiency
;
serum substitution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A water soluble extract from the bark of the Samoan medicinal plantAlphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiencyin vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enhances the cloning efficiency of a T-hybridoma cell line by more than 30 times at otherwise unsuitably low serum concentrations, but does not completely substitute for serum. The enhanced growth of a B-cell hybridoma is also paralleled by an increased production of monoclonal antibodies in cultures containing low cell densities.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00772193
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