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Experiments with Organic Modifiers in Reversed-Flow MEKC (1998)

Vercammen, Joeri ; Verleysen, Katleen ; Sandra, Pat

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 21 (1998), S. 605-607

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ISSN: 0935-6304

Keywords: Reversed-flow MEKC ; separation according to polarity ; organic modifiers ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: ---Reversed-flow MEKC, i.e. MEKC at low pHs to suppress the electroosmotic flow, was evaluated for the separation of some homologous series. At low pHs compared to MEKC, the elution order reverses and normal phase type migration is obtained. Moreover, the elution window is nearly infinite. Efficiencies are very high and reproducibilities in absolute migration times are acceptable (%RSD 〈 5). The influence of a series of alcohol modifiers was investigated.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

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