ISSN:
1573-6881
Keywords:
Cytochromeb structure and function
;
random mutants
;
yeast
;
resistance mutants
;
revertants of deficient mutants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Physics
Notes:
Abstract The generation of random mutations in the mitochondrial cytochromeb gene ofSaccharomyces cerevisiae has been used as a most fruitful means of identifying subregions that play a key role in thebc 1 complex mechanism, best explained by the protonmotive Q cycle originally proposed by Peter Mitchell. Selection for center i and center o inhibitor resistance mutants, in particular, has yielded much information. The combined approaches of genetics and structural predictions have led to a two-dimensional folding model for cytochromeb that is most compatible with current knowledge of the protonmotive Q cycle. A three-dimensional model is emerging from studies of distant reversions of deficient mutants. Finally, interactions between cytochromeb and the other subunits of thebc 1 complex, such as the iron-sulfur protein, can be affected by a single amino acid change.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00762583
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