ISSN:
1432-1424
Keywords:
red cell membranes
;
potassium transport
;
alloimmune antiserum
;
high K-low K polymorphism
;
sheep red cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Antibodies of two specificities in alloimmune sheep anti-L sera, anti-L P and anti-L l , were separated by a new technique and characterized. Absorption of anti-L serum with trypsinized LK (LL) sheep red cells left anti-L P antibodies; the absorbed anti-L l antibodies were then eluted. Anti-L P was only weakly lytic in the presence of complement; it had no effect on passive K influx, but stimulated active K influx. The stimulation could be reversed by eluting the antibody in glycine buffer at low pH. Stimulatory activity in the eluted cells could be restored by resensitization with anti-L P . Anti-L l was more strongly lytic than anti-L P in the presence of complement; it had no effect on active K influx, but inhibited passive K influx. Pig anti-ruminant IgG conjugated to hemocyanin was used to visualize by electron microscopy the number of L P and L l antigen sites onLL sheep red cells sensitized with anti-L P and anti-L l . The values obtained were 590 L P sites/cell and 847 L l sites/cell.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870882
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