ISSN:
0006-3592
Keywords:
microaerobic growth
;
oxygen limitation
;
oxygen uptake
;
recombinant Escherichia coli
;
synthesis and excretion/secretion of α-amylase
;
two-stage culture
;
Vitreoscilla hemoglobin
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Synthesis and excretion of α-amylase is investigated in batch cultures of Escherichia coli JM103[pMK57] (vgb-) and E. coli JM103[pMK79] (vgb+). While total production and excretion of α-amylase were promoted in Luria broth (LB) (excretion being as high as 87%), cell-mass-specific production of the enzyme was promoted in M9 in bioreactor cultures and in LB in shake flask cultures. Low aeration and agitation rates and presence of starch were conducive to α-amylase synthesis in E. coli JM103[pMK79]. Two-stage bioreactor operating strategies that will improve α-amylase production are proposed. The potential of these strategies is demonstrated via two-stage shake flask cultures. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:673-678, 1998.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
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