ISSN:
1573-4986
Keywords:
quail
;
small intestine
;
glycosphingolipids
;
HPLC/FAB/MS
;
immunohistochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Neutral glycosphingolipids were isolated from quail small intestine and their structures were analysed. They contained: Galβ1-4GlcCer(LacCer), Galα1-4GalCer(Ga2Cer), Galα1-4Galβ1-4GlcCer(Gb3Cer), GlcNAcβ1-3Galβ1-4GlcCer(Le3Cer), GalNAcβ1-4Galβ1-4GlcCer(Gg3Cer), GalNAcβ1-4[GalNAcβ1-3]Galβ1-4GlcCer(LcGg4Cer), and GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4GlcCer (Forssman glycolipid) as well as glucosylceramide, galactosylceramide (Nishimura Ket al. 1984)Biochim Biophys Acta 796:269–76) and the Lex glycolipid, III3 Fucα-nLc4Cer (Nishimura Ket al. (1989)J. Biochem (Tokyo) 101:1315–18). The molecular species compositions of these glycosphingolipids were examined using fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography. By such analysis, we could classify the quail glycosphingolipids into at least three classes: glycolipids rich in species having four hydroxyl groups in the ceramides (GalCer, Gg3Cer, LcGg4Cer and Lex), those rich in the ceramides ofN-acyl trihydroxysphinganine with normal fatty acids (Lc3Cer), and glycolipids rich in the ceramides ofN-acyl sphingenine with normal fatty acids (LacCer, Gb3Cer and Forssman glycolipid). Immunohistochemical observation implies that the differences in the hydrophobic moieties specified the localization of glycosphingolipids in the tissue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731151
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