ISSN:
1573-4943
Keywords:
Creatine kinase
;
cyclophilin
;
cyclosporin A
;
protein refolding
;
cis—trans isomerization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A burst phase occurs in the refolding kinetics of guanidine-denatured creatine kinase due to formation of an intermediate within the mixing dead time, with further refolding to the native state after the burst phase along a path following biphasic kinetics. In the presence of cyclophilin, the refolding rates of the two slow processes are accelerated and the values are proportional to the cyclophilin concentration. The activity of cyclophilin in accelerating the slow refolding processes of creatine kinase is totally inhibited by cyclosporin A, indicating that the cis—trans isomerization of the peptidyl—prolyl bonds is involved in the two slow refolding processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007095329303
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