ISSN:
1573-0603
Keywords:
prostate
;
canine
;
primary culture
;
testosterone metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Epithelial-cell enriched primary cultures were established from canine prostate by a collagenase digestion and selective attachment procedure. The epithelial cells in primary culture retained differentiated structure and function. The epithelial cells were attached to one another by tight junctions and desmosomes to form “lumenlike structures” that resemble the acini of the intact organ and appeared to contain typical protein synthetic organelles (1,2). The cultures contained significant levels of acid phosphatase and ornithine decarboxylase (2) and retained the ability to metabolize testosterone to dihydrotestosterone and other 5α-reduced metabolites (2–4).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01665908
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