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  • potyvirus  (1)
  • wheat  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Detection of Potyviral Nuclear Inclusion b Proteins by Monoclonal Antibodies Raised to Synthetic Peptides (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 389-395 
    Details
    ISSN: 1573-8469
    Keywords: potyvirus ; protein overexpression ; RNA-dependent RNA polymerase ; Western blot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Potyviral nuclear inclusion b protein (NIb), the RNA-dependent RNA polymerase, contains three highly conserved regions. Peptides corresponding to these regions were synthesised and used for immunisation. A panel of monoclonal antibodies was obtained. Most of the MAbs reacted with the peptides and a recombinant NIb of PVY in PTA-ELISA. Two of them specifically detected native NIb of potato A, potato V, potato Y, plum pox and turnip mosaic potyviruses in extracts of infected plants in Western blots. Time course experiments revealed that NIb protein can be first detected on the fifth day after infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008763314499
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence of the 3′-terminal region of the RNA of a mite transmitted potyvirus fromHordeum murinum L. (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 123-132 
    Details
    ISSN: 1573-8469
    Keywords: coat protein ; expression ; polymerase ; potyviruses ; sequence ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The 3026 nucleotides upstream of the 3′-polyadenylated tract of a mite transmitted virus fromHordeum murinum L. were cloned and sequenced, and portions of the sequence were expressed inEscherichia coli. Sequence comparisons with wheat streak mosaic virus (WSMV), Agropyron mosaic virus (AgMV) and Hordeum mosaic cirus (HoMV), three mite transmitted potyviruses, and potato virus Y (PVY), the type member of the genusPotyvirus, revealed that the virus is probably a potyvirus, but distinct from WSMV, AgMV, HoMV, and PVY. Serological tests further demonstrated these differences and that the virus is serologically related to another potyvirus, brome streak mosaic virus (BrSMV). We conclude that the virus should be named as the Hordeum isolate of BrSMV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874758
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    Paper (German National Licenses)
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