ISSN:
1573-0972
Keywords:
Acid phosphatase
;
enzyme secretion
;
fungi
;
Neurospora crassa
;
phosphate regulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Both Pi-repressible acid phosphatases, IIb (mycelial) and IIc (extracellular), synthesized by Neurospora crassa and purified to apparent homogeneity by 7.5% PAGE, are monomers, are inhibited by 2 mm ZnCl2 and are non-specifically stimulated by salts. However, the IIc form is activated by p-nitrophenylphosphate (in a negative co-operativity effect with a K 0.5 of 2.5 mm) whereas form IIb shows Michaelis kinetics, with a K m of 0.5 mm. Thus, since both enzymatic forms may be expressed by the same gene (pho-3), it is possible that post-translational modifications lead to the excretion of an enzymatic form with altered Michaelis kinetics compared with the enzymatic form retained by the mycelium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327816
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