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  • phenotypic expression  (1)
  • pluripotent stem cells  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Enzyme-linked immuno-culture assay (1992)
    Springer
    Methods in cell science 14 (1992), S. 31-36 
    Details
    ISSN: 1573-0603
    Keywords: cell culture ; antibody ; HRP ; visualization ; DNA analysis ; phenotypic expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The current study outlines a procedure, designated enzyme-linked immuno-culture assay (ELICA), that will measure phenotypic expression of cultured cells in small plate assays. Given standard curves for phenotypic expression markers and in situ DNA analysis, this procedure will quantitate (to nanogram levels) intracellular, cell surface, or extracellular phenotypic expression markers; visualize the location of the markers; and determine DNA content, all within the same well of a 24- or 96-well tissue culture plate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404545
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of embryonic chick myosatellite and pluripotent stem cells (1992)
    Springer
    Methods in cell science 14 (1992), S. 85-92 
    Details
    ISSN: 1573-0603
    Keywords: isolation ; myosatellite stem cells ; pluripotent stem cells ; myogenic ; uncommitted ; chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The current study outlines the isolation and culture of two populations of cells derived from Day 11 embryonic chick leg muscle and associated connective tissues. The two populations consisted of myogenic lineage-committed stem cells (myosatellite stem cells) and lineage-uncommitted stem cells (pluripotent stem cells). After long-term culture the lineage-uncommitted stem cell population displayed differentiated phenotypes suggestive of the following adult tissues, fibroblasts, muscle, fat, cartilage, and bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404749
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    Paper (German National Licenses)
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