ISSN:
1573-4919
Keywords:
pancreatic β-cells
;
osmotic pressure
;
insulin release
;
regulatory volume decrease
;
potassium permeability
;
tetraethylammonium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract β-Cell-rich pancreatic islets from oblob-mice were used for evaluating the early effects of hypotonic stress. The β-cells responded to an abrupt lowering of the osmotic pressure by 102 mOsm with both a transient stimulation of insulin release (peak value 25 times above basal) and a loss of potassium without major effects on sodium. The secretory response was obtained also in the presence of 100 μM quinine or 20 mM tetraethylammonium+. The loss of potassium was not affected by 20 mM glucose or 10 μM bumetanide, but became less apparent in the presence of 100 μM quinine and disappeared when the islets were exposed to 20 mM tetraethylammonium+. Amiloride and high concentrations of the hypoglycemic sulfonylureas tolbutamide and glibenclamide had only a slight suppressive action on potassium mobilization. Patch clamp analyses revealed an increased frequency of small channel openings after exposure to the hypotonic medium. It is concluded that the pancreatic R-cells have the ability for a regulatory volume decrease involving activation of tetraethylammonium-sensitive K+ conductance. The stimulation of insulin release obtained by lowering the osmotic pressure seems to be related to the entry of water rather than to the ion movements responsible for the readjustment of the β-cell volume.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00228451
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