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  • sturgeon  (3)
  • oral administration  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical investigation of the pituitary of the sturgeon (Acipenser baeri, Chondrostei) (1988)
    Springer
    Fish physiology and biochemistry 5 (1988), S. 109-119 
    Details
    ISSN: 1573-5168
    Keywords: immunohistochemistry ; pituitary ; sturgeon ; chondrostean ; pituitary hormones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the following hormones 1–24 synthetic ACTH (1-24 ACTH), melanophore stimulating hormone (MSH), ovine prolactin (oPRL), ovine growth hormone (oGH), salmon growth hormone (sGH), carp gonadotrophin (cGTH) and its beta subunit (βcGTH), sturgeon gonadotrophin (aciGTH), carp thyrotrophin (cTSH) and β subunit of the human thyrotrophin (βhTSH). The results demonstrate that, in general, the sturgeon pituitary resembles that of teleosts as regards the distribution of the different cell types: ACTH and PRL cells in the rostral pars distalis, GTH, TSH and GH cells in the proximal pars distalis and MSH and PAS-cells in pars intermedia. In addition to the topographical organization of the sturgeon pituitary, this study provides data on the immunological relationships between sturgeon pituitary hormones and those of other vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875699
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  • 2
    Electronic Resource
    Electronic Resource
    Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females (1995)
    Springer
    Fish physiology and biochemistry 14 (1995), S. 313-322 
    Details
    ISSN: 1573-5168
    Keywords: 11KT ; steroidogenesis ; plasma steroids ; ovary ; sturgeon ; Acipenser baeri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00004069
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  • 3
    Electronic Resource
    Electronic Resource
    Plasma kinetics of ingested tritiated estradiol and the influence on estradiol plasma levels in the cultured Siberian sturgeonAcipenser baeri (1991)
    Springer
    Fish physiology and biochemistry 9 (1991), S. 231-245 
    Details
    ISSN: 1573-5168
    Keywords: estradiol ; diet ; oral administration ; metabolism ; sturgeon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasma kinetics of tritiated estradiol (E2) were studied in the Siberian sturgeonAcipenser baeri in order to explain the large amount of E2 found in plasma of 4, 5 and 10-year-old cultured males. This work presents two approaches. The first is based on a single ingestion of3H-labelled E2, which allowed us to plot model curves of resorption and elimination processes. The second deals with chronic ingestion of3H-labelled E2 during a five day period, twice a day, based on a rhythm copied from the feeding practice on a fish farm. Three different doses were tested, based on the amount present in fish diets. Oral administration of E2 to sturgeon leads to E2 accumulation and a saturation of the metabolic processes generally involved in the elimination of aromatic xenobiotics. An explanation of this progressive accumulation of E2 in sturgeon plasma has to take into account the steroid binding proteins. Their synthesis could be induced by the orally administrated E2 and protect it from metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265144
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