ISSN:
0268-2605
Keywords:
Dibutyltin-3-hydroxyflavone
;
fluorescence probe
;
F1F0ATPase
;
tributyltin
;
venturicidin
;
oligomycin
;
DCCD
;
VENR-TETR mutants
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Dibutyltin-3-hydroxyflavone bromide, Bu2Sn(of), is a fluorescent probe inhibitor of mitochondrial F1F0ATPase which reacts with and titrates a component of F0 with marked fluorescence enhancement and reacts similarly with chloroplast CF1CF0 and V-ATPases. Its use to monitor the interactions of other F0 inhibitors (venturicidin, oligomycin, DCCD) with F1F0ATPase, both membrane-bound and purified by solubilization is described. Trialkyltins (Bu3SnCl) back-titrate all Bu2Sn(of) interaction sites; whereas the macrolide inhibitor venturicidin backtitrates 60±5% and oligomycin only 30±3% of Bu2Sn(of) interaction sites. Bafilomycin, the macrolide inhibitor of V-ATPases, is inactive in this assay. DCCD acts in a different fashion from the other inhibitors. Current and potential applications of this fluorescent probe in mitochondrial bioenergetics and biogenesis are discussed.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aoc.590070607
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