ISSN:
1573-5028
Keywords:
Arabidopsis thaliana
;
nucleotide sequences
;
kat genes
;
kinesin-like proteins
;
microtubule-stimulated ATPase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Complementary DNAs of two kinesin-related genes,katB andkatC, were isolated fromArabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long α-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207–754)), which includes the carboxylterminal region of KatC, was expressed inEscherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00028881
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