ISSN:
1573-9368
Keywords:
copper controllable expression
;
metallo-regulatory transcription factor
;
metallo-responsive element
;
nodule-specific expression
;
aspartate aminotransferase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A vector system, based on copper controllable gene expression, has been developed to give control over place as well as time of expression of an introduced gene. This system consists of two elements: (1) the yeastace1 gene encoding a metallo-regulatory transcription factor, ACE1, under control of either an organ-specific or a constitutive promoter; and (2) a gene of interest under control of a chimaeric promoter consisting of the 46 bp TATA fragment of the CaMV 35S RNA promoter linked to four repeats of the ACE1 binding site. The functioning of the system in an organ-specific manner was tested in nodulatedLotus corniculatus plants which consisted of non-transformed shoots plus transformed hairy root tissue ‘wild-type tops/transgenic roots’. After addition of copper ions to the plant nutrient solution, β-glucuronidase (GUS) expression was visualized either specifically in nodules or in both roots and nodules when theace1 gene was placed under control of thenod45 promoter or the CaMV 35S RNA promoter, respectively. The nodule-specific system was used to express antisense constructs of aspartate aminotransferase-P2 in transgenicLotus corniculatus plants. When expression was induced by the addition of copper ions to the plant nutrient solution aspartate aminotranferase-P2 activity declined dramatically, and a decrease of up to 90% was observed in nodule asparagine concentration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01969428
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