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  • 1
    Electronic Resource
    Electronic Resource
    Molecular ecology ofFrankia: Advantages and disadvantages of the use of DNA probes (1991)
    Springer
    Plant and soil 137 (1991), S. 49-54 
    Details
    ISSN: 1573-5036
    Keywords: actinorhizal plants ; nitrogen fixation ; oligonucleotide probes ; ribosomal RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ecological studies on the actinomyceteFrankia are often influenced by the difficulty to isolate and identify this microorganism. The application of molecular biological techniques offers possibilities to detect microbes without isolation and cultivation.Nif genes or whole plasmids can serve as targets for the design of specific probes. Alternatively, ribosomal RNA (rRNA), commonly used in modern phylogenetic studies, can be used as a target molecule in ecological studies. This paper gives an overview of new developments on the use of 16S rRNA as a target molecule for oligonucleotide probes. Group-specific sequences in the 16S rRNA ofFrankia can be used as targets for oligonucleotide probes that a) recognize ineffectiveFrankia strains onAlnus, b) recognize effective strains onAlnus, c) recognize allFrankia strains tested so far. The present paper summarizes the essential steps needed for the use of these probes for the detection ofFrankia strains in soil without isolation and cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187431
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    PCR-amplified 16S rRNA sequence analysis to confirm nodulation of Datisca cannabina L. by the endophyte of Coriaria nepalensis Wall (1994)
    Springer
    Plant and soil 160 (1994), S. 147-152 
    Details
    ISSN: 1573-5036
    Keywords: actinorhizal plants ; cross-inoculation ; Frankia ; nitrogen fixation ; PCR ; 16S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Different Frankia strains and crushed nodule suspensions were tested for their ability to nodulate Coriaria nepalensis and Datisca cannabina. Datisca cannabina seedlings were nodulated effectively by both crushed nodule suspension from Coriaria nepalensis and Datisca cannabina. The origin of the endophyte in Datisca nodules induced by crushed nodules of Coriaria was confirmed by comparing partial PCR-amplified 16S rRNA sequences with those of the endophytes of both plants. Coriaria seedlings could only be nodulated by crushed nodule suspensions of Coriaria nepalensis. All pure cultures of Frankia used as a single inoculum source or in combinations with a nodule filtrate, failed to induce nodulation on Coriaria. Two atypical Frankia strains Cn3 and Cn7 isolated from Coriaria nodules showed no acetylene reduction activity and did not induce nodulation on the host seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00150356
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    Paper (German National Licenses)
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