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Colorimetric determination of the total chlorophyll concentrations in potato leaves by liquid scintillation counting (1987)

Millard, P. ; Robinson, D.

Springer

Potato research 30 (1987), S. 491-494

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ISSN: 1871-4528

Keywords: photon attenuation ; nitrogen fertiliser ; automation ; spectrophotometry

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Summary Extraction of chlorophyll from leaf material with dimethyl sulphoxide allowed measurement of the attenuation of photon detection from a sealed miniature14C standard to be used for total chlorophyll quantification. Comparison between the use of a spectrophotometer and a liquid scintillation counter showed chlorophyll quantification to be accurate by this latter method over a wide range of concentrations. The method does not separate chlorophyllsa andb, but allows the rapid measurement of total chlorophyll concentration, and is suitable for automation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02361926

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