ISSN:
1573-5044
Keywords:
Brassica napus
;
B. juncea
;
mesophyll protoplasts
;
protoplast culture
;
regeneration, rapeseed
;
mustard
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Various factors affecting the culture of Brassica napus and B. juncea mesophyll protoplasts were examined in order to develop suitable culture media for these species. The basic components (salts and vitamins) of culture media K3 and Kao best supported cell division and colony development in protoplast culture of both species. The addition of casamino acids to Kao's medium resulted in colony browning in B. napus genotypes. B. napus protoplasts grew well with glucose as the osmotic stabilizer, whereas B. juncea protoplasts responded better to sucrose. High NAA and low 2,4-D combinations were effective in stimulating colony growth. Colony development was rapid for a range of genotypes cultured with these recommendations in these media and plant regeneration was obtained from protoplast-derived calli in both species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00035906
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