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Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB (2000)

Tanabe, Hideyuki ; Nakagawa, Yuzuki ; Minegishi, Daisuke ; [et al.]

Springer

Chromosome research 8 (2000), S. 319-334

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ISSN: 1573-6849

Keywords: FISH ; human monochromosome hybrid cell panel ; JCRB (Japanese Collection of Research Bioresources) ; mouse A9 cells ; reverse FISH

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The human monochromosome hybrid cell panel in the Japanese Collection of Research Bioresources (JCRB) consists of 23 mouse cell clones, each containing a different human chromosome (the Y chromosome is not yet included). The panel is currently distributed by the Human Science Research Resources Bank (HSRRB) in Osaka. In order to determine the state of the human chromosomes and to supply the information to investigators, we characterized the cells by fluorescence in-situ hybridization (FISH) with corresponding human chromosome-specific painting probes, and, in part, by reverse FISH with the hybrid total DNA hybridized onto human metaphase spreads. Here, we report the frequency of intact human chromosomes maintained in each hybrid and the retained subregions of corresponding human chromosomes with relative frequencies estimated by fluorescent intensity. We used specific painted patterns to classify each hybrid into tentative types with their frequencies showing the nature of each hybrid and the state of rearrangements. This characterization will provide valuable information to investigators using the panel.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1009283529392

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Transfer of a normal human chromosome 11 suppresses tumorigenicity of some but not all tumor cell lines (1990)

Oshimura, Mitsuo ; Kugoh, Hiroyuki ; Koi, Minoru ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 42 (1990), S. 135-142

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ISSN: 0730-2312

Keywords: tumor-suppressor gene ; microcell-fusion ; pSV2-neo ; nude mouse ; mono-chromosome ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: The complete suppression of tumorigenicity of a human cervical cancer cell (HeLa) and a Wilms' tumor cell line (G401) following the introduction via microcell fusion of a single chromosome t(X;11) has been demonstrated by Stanbridge and co-workers. To determine whether other tumor cell lines are suppressed by chromosome 11, we performed chromosome transfer experiments via microcell fusion into various human tumor cell lines, including a uterine cervical carcinoma (SiHa), a rhabdomyosarcoma (A204), a uterine endometrial carcinoma (HHUA), a renal cell carcinoma (YCR-1), and a rat ENU-induced nephroblastoma (ENU-T1). We first isolated a mouse A9 cell containing a single human chromosome 11 with integratedp SV2-neo plasmid DNA. Following microcell fusion of the neo-marked chromosome 11 with the various tumors mentioned above, we isolated clones that were resistant to G418 and performed karyotypic analyses and chromosomal in situ hybridization to ensure the transfer of the marked chromosome. Whereas the parental cell of each cell line were highly tumorigenic, SiHa and A204 microcell hybrid clones at early passages were nontumorigenic in nude mice and HHUA was moderately tumorigenic. On the other hand, YCR-1 and ENU-T1 microcell hybrid clones were still highy tumorigenic following the introduction of chromosome 11. Thus, the introduction of a normal chromosome 11 suppresses the tumorigenicity of some but not all tumors, suggesting that the function of the putative suppressor gene(s) on chromosome 11 is effective only in specific tumors.

Additional Material: 1 Ill.