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  • 1
    Electronic Resource
    Electronic Resource
    Adsorption of trypsin onto styrene-2-hydroxyethyl methacrylate copolymer microspheres and its enzymatic activity (1986)
    Springer
    Colloid & polymer science 264 (1986), S. 743-747 
    Details
    ISSN: 1435-1536
    Keywords: Emulsion polymerization ; latex ; microsphere ; enzyme ; immobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Effects of the surface hydrophilicity of styrene-2-hydroxyethyl methacrylate (S-HEMA) copolymer microspheres on the adsorption immobilization of trypsin and on its enzymatic activity were discussed. In the case of a polystyrene (PS) microsphere, the amount of adsorption was large, whereas the enzymatic activity of the adsorbed trypsin was low. On the other hand, in the case of an S-HEMA copolymer microsphere whose HEMA content was 10 mole %, the maximum specific activity of trypsin adsorbed was nearly equal to that of free trypsin, although the amount of adsorption decreased to about 65 % of that in PS. Accordingly, total activity, which is the product of the amount of adsorption and the specific activity, could be enhanced by controlling the surface hydrophilicity of the microsphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01500748
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of carboxyl groups at surface layer of carboxylated polymer emulsion particles by alkali treatment (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 246-249 
    Details
    ISSN: 1435-1536
    Keywords: Polymer emulsion ; latex ; carboxyl group ; microsphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract For the purpose of localizing carboxyl groups from inside to particle surface, styrene — butyl acrylate — methacrylic acid (74.3/17.0/8.7, mol ratio) terpolymer emulsion was kept under pH 9 at different temperatures. The amount of carboxyl groups at the particle surface, As, was remarkably increased by the alkali-treatment above 35 °C. On the other hand, As value of the alkalitreated emulsion was decreased by keeping under ph 3 above 45 °C, although in the case of the original emulsion without the alkalitreatment, it was not changed by the acid-treatment. These results suggest that a part of polymer segments which have ionized carboxyl groups is dragged out at the surface by an increase in the affinity of the groups against water, and the dragged segments turn back into the inside again when the carboxyl groups are deionized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01412714
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  • 3
    Electronic Resource
    Electronic Resource
    Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/polystyrene composite microspheres by cyanogen bromide method and its enzymatic activity (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 957-964 
    Details
    ISSN: 1435-1536
    Keywords: Enzymeimmobilization ; trypsin ; cyanogen bromide ; emulsion ; microsphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Covalent immobilization of trypsin onto poly(2-hydroxyethyl methacrylate)/ polystyrene composite microspheres, produced by emulsifier-free seeded emulsion polymerization technique, was carried out using the cyanogen bromide method under various conditions. The highest enzymatic activities of the trypsin immobilized in this study against N-α-benzoyl-L-arginine ethyl ester and N-α-benzoyl-L-arginine-p-nitroanilide, as low-molecular substrates and casein as a high-molecular substrate, were high, as corresponding to 80%, 85% and 50% of those of free trypsin, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01412397
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  • 4
    Electronic Resource
    Electronic Resource
    Production of anomalously shaped carboxylated polymer particles by seeded emulsion polymerization (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 876-881 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; anomalous particle ; microsphere ; latex ; emulsionpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Anomalous polymer particles with a partial protuberance like “octopus ocellatus” were produced under alkaline conditions by seeded emulsion copolymerization for styrene and butyl acrylate, with styrene-butyl acrylate-methacrylic acid terpolymer emulsion as seed. The mechanism of production of the polymer particles was studied. By transmission electron microscopic observation of the particles at each conversion, it was observed that the anomalous polymer particles were produced by partial growth of each of the individual seed particles throughout polymerization. Ionization of the carboxyl groups and low viscosity in the growing particles during the process of polymerization were important factors for partial growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01421815
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