ISSN:
0006-3592
Keywords:
SDS-alkaline lysis process
;
lysis reaction time
;
chromosomal DNA denaturation
;
shear damage of DNA
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
SDS-alkaline lysis of recombinant Escherichia coli cell suspensions was carried out in a coaxial cylinder rheometer, and the data were used to establish the time course of lysis reaction. The results of the experiments showed that cell lysis reaction time depended on cell strain but was unaffected by plasmid size and plasmid copy number. The high molecular weight globular proteins and chromosomal DNA were denatured, and the resulting changes in rheometric measurements characterised the denaturation time. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 768-770, 1998.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
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