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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 110 (1999), S. 127-132 
    ISSN: 1573-5060
    Keywords: AFLP ; chiasma localisation ; leek ; linkage map ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic linkages of 1:1 segregating amplified fragment length polymorphism (AFLPTM) markers in leek (2n=4x=32) were studied with the aim of detecting block inheritance resulting from strong suppression of crossing over in chromosomal arms. The study comprised 97 markers in 70 plants originating from a cross between two highly heterozygous parents. At LOD≥3, 52 markers were linked in 23 linkage groups, spanning 405 of an expected 6400 cM. Cytogenetic data in previous studies indicated that recombination may be suppressed in two-thirds of the leek genome, supposedly leading to large linkage blocks of markers. In the present study, however, comparison of AFLP linkages with simulations indicates that the linkage blocks are much smaller, comprising no more than one-third of the leek genome.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Allium porrum ; autotetraploidy ; introgression ; Phytophthora porri ; resistance ; screening methods ; leek
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new screening method was developed to evaluate resistance of leek (Allium porrum) to Phytophthora porri, based on inoculation by 24 h-immersion of leek plantlets in the 3–6 leaf stage in a suspension of ca. 100 zoospores.ml-1. The immersion test was used for identifying new sources of resistance and to determine the genetic basis of resistance. Within winter leek, five resistance classes were defined on the basis of average field scores of 21 plants. Clones from these plants were tested with the immersion technique. The average scores per resistance class in immersion and field tests were significantly correlated (P〈0.01). The correlation of single-date field scores with the immersion test scores was better in the second half of the epidemic season. A selection experiment yielded a strong response to selection for resistance (53–97%) but no response to selection for susceptibility. This may indicate that resistance is due to few recessive genes in the studied winter leek. Crosses between landraces and winter leek were analyzed by means of F2 (selfed F1) and BC1, progenies. This analysis indicated the presence of few loci with dominant genes for resistance in accession CGN 873243, and additive polygenes in accession Pl 368351.
    Type of Medium: Electronic Resource
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