ISSN:
1573-5087
Keywords:
inflorescence explants
;
in vitro micropropagation
;
medicinal plant
;
Ocimum sanctum
;
plant conservation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract In vitro micropropagation of holy basil (Ocimum sanctum L.), an Indian medicinal herb, has been accomplished on Murashige and Skoog (MS) medium utilizing young inflorescence explants. MS supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or thidiazuron (TDZ) produced only non-morphogenetic callus. Direct multiple shoots differentiated within 2--3 weeks when explants were cultured on MS containing 6-benzyl aminopurine (BAP). Of the various levels of BAP tested, MS + BAP (1.0 mgl−1) produced the maximum number of shoots. Incorporation of indole-3-acetic acid (IAA) (0.05 mgl−1) along with BAP (1.0 mgl−1) in the culture medium showed a marked increase in the number of shoots. About 92% of the in vitro regenerated shoots rooted on MS hormone-free medium within 2--3 weeks of culture and 85% of the micropropagated plantlets could be successfully established in soil, where they grew normally.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006201631824
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