ISSN:
1573-6881
Keywords:
(Na+ + K+)-ATPase
;
(K+ + H+)-ATPase
;
goat antibodies
;
immunoblotting
;
immunological cross-reactivity
;
enzyme inhibition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Physics
Notes:
Abstract Goat antisera against (Na+ + K+)-ATPase and its isolated subunits and against (K+ + H+)-ATPase have been prepared in order to test for immune cross-reactivity between the two enzymes, whose catalytic subunits show great chemical similarity. None of the (Na+ + K+)-ATPase antisera cross-reacted with (K+ + H+)-ATPase or inhibited its enzyme activity. The same was true for the (K+ + H+)-ATPase antiserum with regard to (Na+ + K+)-ATPase and its subunits and its enzyme activity. So not withstanding the chemical similarity of their subunits, there is no immunological cross-reactivity between these two plasma membrane ATPases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00751051
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