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  • hippocampus  (2)
  • immortalization  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Establishment and characterization of immortalized hippocampal neural precursor cell lines (2000)
    Springer
    Cytotechnology 33 (2000), S. 53-61 
    Details
    ISSN: 1573-0778
    Keywords: brain development ; brain repair ; hippocampus ; immortalization ; Myc ; neural precursor cells ; p53
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In the mammalian central nervous system, a complexcircuit of neurons contributes to higher behaviors.Each region of the brain has a unique function derivedfrom various types of neurons. Several neuralprecursor cell lines have been established from basalganglia of fetal brain. In this study, hippocampalneural precursor cell lines were established from thehippocampus of p53-/- embryos. By means ofintegration of a MycER regulatable oncoprotein intop53-/- neural precursor cells, several immortallines were established from embryonic hippocampalprimordium, with bFGF and estrogen continuouslysupplied for activation of the MycER protein. A dualluciferase study demonstrated that the MycER proteinblocked the expression of a glial cell marker protein,GFAP, probably contributing to the persistent celldivision of the immortalized neural precursor cells.These cell lines differentiate into neuronal and glialcell types after withdrawal of bFGF. The phenotype ofthe hippocampal cell lines differed from that of thebasal ganglia cell lines as observed in a clonaldensity culture. This result implies that each regionof the brain has a unique developmental program, thatmay be imprinted in each of the neural precursor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008159211932
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  • 2
    Electronic Resource
    Electronic Resource
    Glutamate triggers elevation of intracellular Ca2+ concentration in neural precursor cells (2000)
    Springer
    Cytotechnology 33 (2000), S. 157-165 
    Details
    ISSN: 1573-0778
    Keywords: basal ganglia ; elevation of intracellular Ca2+ concentration ; glutamate ; hippocampus ; immortalized cell lines ; neural precursor cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Both neurons and glial cells are derived from neuralprecursor cells in the ventricular zone during braindevelopment. The fate of the neural precursor cells isaffected by neurotransmitters such as glutamate. Inthis study, we examined glutamate-triggeredintracellular Ca2+ signaling in neural precursorcell lines by the calcium digital imaging method. Whenimmortalized primary-cultured neural precursor cellswere treated with glutamate, a subpopulation of thesecells showed an increase in intracellular Ca2+concentration. In an effort to determine the role ofthe glutamate-triggered intracellular Ca2+ signalin neural precursor cells, we tried to cultureimmortalized basal ganglial and hippocampal neuralprecursor cell lines in glutamate-free medium. Thehippocampal (MHP-2) cells became adapted to theglutamate-free medium, and when treated with glutamatethe adapted subline (MHP-2-E1) showed an increase inintracellular Ca2+ concentration. In contrast,the basal ganglial neural precursor cell lines failedto become adapted to the glutamate-free medium. Theseresults suggest that hippocampal and basal ganglialneural precursor cells differ in their cellularresponse to glutamate as an exogenous stimulus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008102621059
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