ISSN:
1573-904X
Keywords:
high-performance liquid chromatography
;
enantiomeric purity
;
optical purity
;
enantiomeric separation
;
ephedrine
;
pseudoephedrine
;
precolumn derivatization
;
S( + )-1-(1-naphthyl)-ethyl isocyanate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract An HPLC method has been developed for the separation of four stereoisomers of ephedrine using precolumn derivatization with S( + )-l-(l-naphthyl)-ethyl isocyanate. The formed derivatives are subsequently separated on a normal-phase column and are detected at a UV wavelength of 220 nm. This method was used to quantitate the differences in the enantiomeric impurity of various samples of ( + )-pseudoephedrine. The reported method can differentiate between samples of ( + )-pseudoephedrine which differ in their enantiomeric impurity by as little as 0.02%. Possible racemization of ( + )-pseudoephedrine in aqueous solutions was also studied. Samples of ( + )-pseudoephedrine from various suppliers and, indeed, different lots from the same supplier, differed significantly in their degree of enantiomeric impurity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1015813509816
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