ISSN:
1573-5044
Keywords:
antifungal agent
;
callus
;
fungal contamination
;
hairy roots
;
miconazole
;
shoot cultures
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Miconazole at concentrations between 5 and 20 mgl-1 inhibited hyphal growth and sporulation in a wide range of fungi commonly associated with plants. These fungi included 4 Oomycetes, 11 Deuteromycetes, 4 Ascomycetes and 4 common airborne contaminants of plant tissue culture. The phytotoxicity of 20 mgl-1 miconazole was also tested against a wide range of in vitro plant cultures. Shoot cultures from 15 species showed either no response or a slight growth reduction in the presence of miconazole. Although the growth of shoot cultures in 2 other species was significantly reduced by miconazole, a positive growth rate was maintained. Callus and hairy root cultures from 5 species were more sensitive to miconazole than shoot cultures, although they also retained a positive growth rate in the presence of the antifungal agent. Reconstruction experiments demonstrated the effectiveness miconazole had for the rescue of plant material from in vitro cultures contaminated from fungi.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042292
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