ISSN:
1617-4623
Keywords:
gdhA1 point mutation
;
Deletion analysis
;
Recombination frequency
;
PCR
;
DNA sequencing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract gdhA1 is a spontaneous mutant of Escherichia coli that causes complete loss of activity of the NADP-specific glutamate dehydrogenase (GDH) encoded by the gdhA gene. The gdhA1 mutational site has been identified by recombinational mapping, polymerase chain reaction (PCR) amplification and DNA sequencing, as an A to G transition at nucleotide 274 of the gdhA coding sequence, resulting in an amino acid change of lysine 92 to glutamic acid. The mutant enzyme forms hybrid hexamers with a wild-type GDH, providing a useful system for analysis of conformational integrity of mutational variants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00277068
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