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  • enzyme-linked immunosorbent assay  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 14 (1992), S. 25-29 
    ISSN: 1573-0603
    Keywords: Idiotypic antibodies ; rheumatoid factor/idiotypic antibody immune complexes ; anti-idiotypic antibodies ; enzyme-linked immunosorbent assay ; immunoaffinity isolation ; B cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Techniques are described for the production of anti-idiotypic B cells by immunization with idiotypic antibodies complexed to rheumatoid factor. Screening for anti-idiotypic antibody production and immunoaffinity isolation of the B cells, actively producing the anti-idiotype antibody, by idiotypic antibody-coated magnetic beads is described. This methodology can be used to isolate anti-idiotypic B cells for immune regulation studies or to provide fusion partners for the production of monoclonal anti-idiotypic antibodies.
    Type of Medium: Electronic Resource
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