ISSN:
1573-0603
Keywords:
Idiotypic antibodies
;
rheumatoid factor/idiotypic antibody immune complexes
;
anti-idiotypic antibodies
;
enzyme-linked immunosorbent assay
;
immunoaffinity isolation
;
B cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Techniques are described for the production of anti-idiotypic B cells by immunization with idiotypic antibodies complexed to rheumatoid factor. Screening for anti-idiotypic antibody production and immunoaffinity isolation of the B cells, actively producing the anti-idiotype antibody, by idiotypic antibody-coated magnetic beads is described. This methodology can be used to isolate anti-idiotypic B cells for immune regulation studies or to provide fusion partners for the production of monoclonal anti-idiotypic antibodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404544
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