ISSN:
1040-0397
Keywords:
Lactate
;
malate
;
wine
;
flow injection analysis
;
enzyme reactor
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The simultaneous determination of L-malate and L-lactate, by enzyme-supported flow injection analysis (FIA), was developed using two enzyme reactors in parallel and a single oxygen electrode. NADH formed in the reaction of malate dehydrogenase (MDH) was regenerated to NAD+ with dissolved oxygen, using vitamin K3 and diaphorase (DI). L-Lactate was determined using the enzyme lactate oxidase (LOD). When sample solutions were simultaneously injected into the two reactors (the MDH-DI-reactor and the LOD-reactor) with a controlied residence time, a “train” of two peaks corresponding to L-lactate and L-malate were seen in the FIA-gram. The peak currents were linearly related to the L-malate and L-lactate concentration in the range 0.05-1.2 mM and 0.01-0.5 mM, respectively. The present system was applied to the determination of L-lactate and L-malate in wine. The results showed good agreement with those obtained using a conventional method (F-kit method), suggesting that this system may be applicable to the monitoring of malo-lactic fermentation during wine production.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140040506
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