ISSN:
1573-904X
Schlagwort(e):
γ-cyclodextrin
;
enzyme immunoassay
;
acetylcholin-esterase
;
pharmacokinetic analysis
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Abstract Purpose. Because of its ability to form complexes with drugs, γ-cyclodextrin is of great potential value in pharmaceutical formulations. The biological fate of γ-cyclodextrin must therefore be considered in safety evaluation, using sensitive and specific methods applicable to biological fluids. Methods. Antibodies were raised against γ-cyclodextrin, allowing the development of a new enzyme immunoassay. The analytical characteristics of this assay were evaluated. Rats were given a single intravenous 25 mg/kg dose of γ-cyclodextrin. Plasma and urine samples were collected and assayed. Results. This new enzyme immunoassay was sensitive (limit of detection close to 94 pg/mL) and suitable for quantification of γ-cyclodextrin in urine and plasma after methanol extraction. The use of different linear and cyclic compounds demonstrated the high specificity of the assay. After i.v. administration, the concentration of γ-cyclodextrin rapidly decreased in the plasma while the molecule was probably distributed into the tissues. Although urinary elimination predominates, only 50% of the injected γ-cyclodextrin was recovered in urine, suggesting enzymatic degradation and/or tissular storage. Conclusions. This assay may provide important information on the fate of γ-cyclodextrin inclusion complexes dedicated to drug-delivery using various modes of administration (oral, parenteral, transmucosal or dermal).
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1018903109564
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