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A rapid enhanced chemiluminescent immunoassay for allergen-specific IgE antibodies (1986)

Stiles, Heather J. ; Bicknell, Helen J. ; Matthews, Jayne A.

New York : Wiley-Blackwell

Journal of Bioluminescence and Chemiluminescence 1 (1986), S. 53-58

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ISSN: 0884-3996

Keywords: enhanced chemiluminescence ; immunoassay ; allergen ; IgE ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: An enhanced chemiluminescent immunoassay is described for the measurement of allergen-specific IgE antibodies. The assay is demonstrated for pollen from four grass species. A comparison was made between results obtained by this method and those obtained by the radioallergosorbent (RAST) procedure; a high degree of correlation (r = 0.95) was found for D. glomerata specific IgE. The assay is rapid and can be carried out in under 1 hour. The advantages of the luminescent assay as compared with the RAST procedure are discussed.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bio.1170010203

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