ISSN:
1573-5060
Keywords:
Glycine max
;
Glycine tabacina
;
Glycine tomentella
;
soybean
;
interspecific hybrid
;
wide hybridization
;
embryo culture
;
embryo rescue
;
culture media
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Immature embryos of Glycine max (L.) Merr. cv. ‘Bay’, G. tabacina (Labill.) Benth. and G. tomentella Hayata were cultured on 72 media combinations to identify a nutrient medium which would allow a greater percentage of interspecific plants to be recovered from cultured embryos of G. max × various perennial Glycine species. The highest mean plant recovery rate of 79% was from a medium containing ‘B5’ nutrient salts as reported by Gamborg et al. (1968), vitamin components according to Williams (1978) and 30 g/l sucrose. This is as compared with 25% from a medium used previously. In an additional test, 67% of hybrid embryos of G. max × G. tomentella were recovered from the same medium, from which G. tomentella was most effectively recovered in all testing.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00023635
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