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  • 1
    Electronic Resource
    Electronic Resource
    Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response (1995)
    Springer
    Plant molecular biology 28 (1995), S. 885-900 
    Details
    ISSN: 1573-5028
    Keywords: alfalfa ; elicitation ; glucose 6-phosphate dehydrogenase ; isoflavonoids ; pentose phosphate pathway ; 6-phosphogluconate dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding genes from bacteria, Drosophila and mammals. Transcripts encoding both enzymes are expressed at high levels in roots and nodules. Exposure of alfalfa suspension cells to an elicitor from yeast cell walls results in co-ordinated increases in transcription rates for both genes, followed by increased steady state transcript levels but only slightly increased extractable enzyme activities, at the onset of accumulation of isoflavonoid phytoalexins. Levels of NADPH and NADP remain relatively constant in alfalfa cells following elicitation. The rapid transcriptional activation of 6PGDH and G6PDH does not therefore appear to be a response to altered pyridine nucleotide redox state. These genes appear to respond to early events in elicitor-mediated signalling rather than to subsequent elicitor-induced changes in secondary metabolism. Hydrogen peroxide, a potential signal for elicitation of anti-oxidative genes in biologically stressed plant cells, did not induce 6PGDH or G6PDH transcripts or enzymatic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042073
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  • 2
    Electronic Resource
    Electronic Resource
    Stress responses in alfalfa (Medicago sativa L.). XX. Transcriptional activation of phenylpropanoid pathway genes in elicitor-induced cell suspension cultures (1996)
    Springer
    Plant molecular biology 30 (1996), S. 427-438 
    Details
    ISSN: 1573-5028
    Keywords: elicitation ; gene transcription ; isoflavonoid phytoalexins ; Medicago sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclear transcript run-on analysis was used to investigate the relative transcription rates of genes encoding enzymes of isoflavonoid phytoalexin biosynthesis and related pathways in elicitor-treated alfalfa (Medicago sativa L.) cell suspension cultures. Genes encoding L-phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and chalcone reductase (CHR) were most rapidly activated, with increases in transcription measurable within 10–20 min after elicitation. Cinnamic acid 4-hydroxylase (C4H), chalcone isomerase (CHI), isoflavone reductase (IFR) and caffeic acid 3-O-methyltransferase (COMT) genes were also rapidly activated, but at a slower initial rate. Transcription of chalcone 2′-O-methyltransferase (CHOMT), and 1,3-β-D-glucanase genes was less rapid, with lag periods of 60 and 30 min post-elicitation, respectively. Treatment of cells with the PAL inhibitor L-α-aminooxy-β-phenylpropionic acid (AOPP) resulted in increased transcription of PAL, CHS and CHR, but reduced transcription of CHOMT, indicating a role for phenylpropanoid products as both negative and positive regulators of gene expression within the phenylpropanoid pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049322
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