ISSN:
0021-9304
Keywords:
fiber-optic pH sensor
;
pH sensing membrane
;
fluorescent probe
;
SNAFL
;
photo-crosslinking
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
A fiber-optic pH sensor was prepared using the self-referencing fluorescent pH probe carboxy seminaphthofluorescein (SNAFL-2). The ratio of the emission from the base form of this dye and the emission near the isoemissive point is insensitive to variations in the excitation intensity and photobleaching. The carboxy SNAFL-2 and a photoaffinity crosslinker, 4-azido-2,3,5,6-tetrafluorobenzoic acid, succinimidyl ester, were attached onto poly(acrylamide-co-vinylamine) to form a hydrophilic functional membrane for the fiber-optic sensor. Photo crosslinking was used to create a crosslinked pH-sensing membrane and covalently bind the membrane onto the surface of the PMMA optical fiber. The fluorescent properties of the membrane-fiber conjugate have been determined. The membrane is stable, and the pH sensor shows a fast response time and excellent resolution in a wide pH range of 3 to 11. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 9-15, 1998.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
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