ISSN:
1573-0603
Keywords:
alcelaphine herpesviruses
;
malignant catarrhal fever
;
cell culture
;
electron microscopy
;
virus isolation
;
serologic methods
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Cultures of fetal aoudad sheep kidney (FAK), bovine embryonic lung (BEL), and African green monkey kidney (Vero) cells were compared for differential replication of alcelaphine herpesviruses. Cell-free virus appears more rapidly when infected cells are incubated at 33° C rather than at 37° C. Events in the replication and morphologic development of several alcelaphine herpesvirus isolates have been documented using light and electron microscopy. Techniques for indirect immunofluorescence and serum virus neutralization are described. When virus free of host-cell contaminants is desired for biochemical analysis, virus isolates are initially purified on sucrose gradients and then further purified by density gradient centrifugation in Percoll.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404132
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