ISSN:
1573-0972
Keywords:
Amylase
;
cellulase
;
ectomycorrhizal fungi
;
β-glucosidase
;
lignin degrading enzymes
;
lipase
;
lyophilization
;
urease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Freeze-dried ectomycorrhizal fungus cultures were tested for qualitative enzymatic activity and compared with that of the non-lyophilized culture. Enzymes involved in the utilization of starch, cellulose, lipid, lignin and urea were tested for their qualitative presence/activity. Expression of amylase and urease was stronger than that of lipase and lignin-degrading activity for the isolates tested. Variation among the species of Laccaria was low and prominently seen only for cellulase and urease. Amanita muscaria showed significant variation relative to other members of the Agaricales reported in the present study, except for β-glucosidase activity. All the enzymatic tests showed an unequivocal uniformity between the lyophilized vegetative mycelium (L) and the respective non-lyophilized mycelial cultures (NL), indicating that the lyophilization procedure maintained stable enzyme activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008921419630
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