ISSN:
1420-9071
Keywords:
Molting hormone
;
3-epi-ecdysteroids
;
ecdysteroid conjugate
;
silkworm
;
Bombyx mori
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Ecdysteroids in diapause eggs of the silkworm,Bombyx mori, were analyzed using high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). A relatively large amount of an unidentified free ecdysteroid and its phosphoric ester (conjugated form) were detected. These two compounds were isolated by a combination of column chromatography on silicic acid, thin-layer chromatography (TLC), and HPLC using a reverse-phase (RP) column. The purified compounds were identified as 3-epi-22-deoxy-20-hydroxyecdysone (22d20E′) and 3-epi-22-deoxy-20-hydroxyecdysone 2-phosphate (22d20E′2P) by means of mass spectrometry and nuclear magnetic resonance spectroscopy. to our knowledge, this is the first report of 22d20E′ and 22d20E′2P.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01928896
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