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  • 1
    ISSN: 1572-9699
    Keywords: sulfate reduction ; Desulfotomaculum ; thermophily ; fatty acids degradation ; autotrophy ; complete oxidation ; hydrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strictly anaerobic, thermophilic, fatty acids-degrading, sporulating sulfate-reducing bacterium was isolated from geothermal ground water. The organism stained Gram-negative and formed gas vacuoles during sporulation. Lactate, ethanol, fructose and saturated fatty acids up to C18 served as electron donors and carbon sources with sulfate as external electron acceptor. Benzoate was not used. Stoichiometric measurements revealed a complete oxidation of part of butyrate although growth with acetate as only electron donor was not observed. The rest of butyrate was oxidized to acetate. The strain grew chemolithoautotrophically with hydrogen plus sulfate as energy source and carbon dioxide as carbon source without requirement of additional organic carbon like acetate. The strain contained a c-type cytochrome and presumably a sulfite reductase P582. Optimum temperature, pH and NaCl concentration for growth were 54°C, pH 7.3–7.5 and 25 to 35 g NaCl/l. The G+C content of DNA was 50.4 mol %. Strain BSD is proposed as a new species of the spore-forming sulfate-reducing genus Desulfotomaculum, D. geothermicum.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 63-68 
    ISSN: 0006-3592
    Keywords: dissolved-hydrogen probe ; anaerobic digestion ; hydrogen ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The concentration of diatomic hydrogen in the liquid phase of an anaerobic digester was used to determine the onset of digester failure induced by substrate overloading. The construction of an inexpensive probe to measure dissolved hydrogen, having a partial pressure detection limit of 30 Pa, is described. An increase in the partial pressure of dissolved hydrogen, from less than 30 Pa to 400 Pa, was observed when the D-glucose concentration in a laboratory-scaled digester was increased rapidly to 10 mM. However, when the digester was gradually overloaded, an increase in the dissolved-hydrogen partial pressure was not observed until after the digester failed. The accumulation of volatile fatty acids and digester failure were observed at dissolved-hydrogen partial pressures below 30 Pa. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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