ISSN:
1573-5168
Keywords:
Platichthys stellatus
;
creatine
;
turnover
;
phosphocreatine
;
creatinine
;
white muscle
;
liver
;
gills
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Starry flounder (Platichthys stellatus) were cannulated and a bolus of 9 μCi14C-creatine in saline was injected into the caudal vein. The fish were sacrificed at intervals ranging from 1h to 36d after label injection. Creatine pool size (PCr+Cr) and creatinine (Crn) content in blood, muscle, gills and liver were analyzed and specific activities (SA) determined. Mean concentrations of PCr+Cr/Crn in PCA-extracts of muscle, gills, liver and blood of experimental fish (at rest) were 38.1/2.40, 4.1/0.25, 5.6/0.45 and 0.3/n.d. μmol.g−1 respectively. Within 10 min, plasma SA had decreased by approximately 90%. In white muscle, the rate of14C−Cr appearance as well as label disappearance was slow compared to gills and liver. In fish examined 36d postinjection, mean SA in muscle had decreased to 23% of maximum SA which occurred 24h after injection.14C−Cr was incorporated into the liver tissue at a very high rate, SA being two orders of magnitude higher in liver than in white muscle. Over the first 6d, retention of label was observed in liver; after 36d only 3% of the original label was detected. Creatine pool size (PCr+Cr) in white muscle decreased with food deprivation. In flounder sacrificed after 36d, PCr+Cr was only 52% that of fed control fish, suggesting that creatine or precursors for its biosynthesis are supplied with the diet.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875599
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