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  • 1
    Electronic Resource
    Electronic Resource
    Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB (2000)
    Springer
    Chromosome research 8 (2000), S. 319-334 
    Details
    ISSN: 1573-6849
    Keywords: FISH ; human monochromosome hybrid cell panel ; JCRB (Japanese Collection of Research Bioresources) ; mouse A9 cells ; reverse FISH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The human monochromosome hybrid cell panel in the Japanese Collection of Research Bioresources (JCRB) consists of 23 mouse cell clones, each containing a different human chromosome (the Y chromosome is not yet included). The panel is currently distributed by the Human Science Research Resources Bank (HSRRB) in Osaka. In order to determine the state of the human chromosomes and to supply the information to investigators, we characterized the cells by fluorescence in-situ hybridization (FISH) with corresponding human chromosome-specific painting probes, and, in part, by reverse FISH with the hybrid total DNA hybridized onto human metaphase spreads. Here, we report the frequency of intact human chromosomes maintained in each hybrid and the retained subregions of corresponding human chromosomes with relative frequencies estimated by fluorescent intensity. We used specific painted patterns to classify each hybrid into tentative types with their frequencies showing the nature of each hybrid and the state of rearrangements. This characterization will provide valuable information to investigators using the panel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009283529392
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  • 2
    Electronic Resource
    Electronic Resource
    Cosmids and transcribed sequences from chromosome 11q23 (1995)
    Springer
    Journal of human genetics 40 (1995), S. 307-317 
    Details
    ISSN: 1435-232X
    Keywords: chromosome 11q23 ; radiation-reduced hybrid ; cosmids ; exon amplification ; transcribed sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome 11 was prepared from microcell hybrid A9 (neo11) cells containing a normal human chromosome 11 tagged with pSV2neo at 11p11.2. Among 15 radiation hybrid clones isolated, RH(11)-9 which contains a q23 fragment in addition to theneo integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 11q23 and 6 cosmids to 11p11.2. Exon amplification proceeded with 23 of the 59 cosmids and 16 putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH-(11)-9 cells and putative exons also facilitate the positional cloning of genes in the 11q23 region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01900597
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